全部商品分类
实验耗材
生物仪器
分子生物
细胞生物
蛋白因子
药物研发
细胞治疗
样本储藏
培养基
试剂盒
抗体
测序试剂盒
您的浏览历史
您现在的位置: 首页 > 分子生物 > ELISA试剂盒 > Human TNF-α Pre-Coated ELISA Kit 人TNF-Alpha ELISA试剂盒

Human TNF-α Pre-Coated ELISA Kit 人TNF-Alpha ELISA试剂盒

Human TNF-α Pre-Coated ELISA Kit 人TNF-Alpha ELISA试剂盒
  • Human TNF-α Pre-Coated ELISA Kit 人TNF-Alpha ELISA试剂盒
英文名称: Human TNF-α Pre-Coated ELISA Kit
产品应用: TNF-α对多种肿瘤细胞具有细胞毒性,是介导细菌感染免疫应答的重要因素。TNF-α在感染性休克,自身免疫性疾病,类风湿性关节炎,炎症和糖尿病的诱导中也起了重要作用
中国俗称: TNF-α ELISA试剂盒 中国简称: TNF-α ELISA kit
注册品牌: Peprotech/Biogems 生产厂家: Peprotech/Biogems
订购货号: BGK01375
规格型号: 96T
保存温度: 4-8度
产品种类: ELISA kit
到货时间: 现货1~5天
产品编码: YS0010145
市场价格: ¥0元
本店售价: ¥0元
注册用户: ¥0元
金牌用户: ¥0元
购买数量: 单价单位

产品描述

产品中文名称:人TNF-Alpha ELISA试剂盒

产品英文名称:Human TNF-α Pre-Coated ELISA Kit

品牌:Peprotech/Biogems

货号:BGK01375
购买Peprotech/Biogems ELISA试剂盒送精美礼品一份!
上海华雅思创生物科技有限公司代理的Peprotech/Biogems TNF-Alpha ELISA试剂盒的产品描述:

TNF-α是由各种细胞分泌的多效促炎细胞因子,包括脂肪细胞,活化的单核细胞,巨噬细胞,B细胞,T细胞和成纤维细胞。它属于TNF家族的配体,并通过两种受体TNFR1和TNFR2发出信号。TNF-α对多种肿瘤细胞具有细胞毒性,是介导细菌感染免疫应答的重要因素。TNF-α在感染性休克,自身免疫性疾病,类风湿性关节炎,炎症和糖尿病的诱导中也起了重要作用。人和鼠TNF-α表现出显着的物种间反应性。TNF-α存在两种形式; II型跨膜蛋白和成熟可溶性蛋白。蛋白水解切割TNF-α跨膜蛋白以产生可溶性,生物活性的17kDa TNF-α,

BioGems人类TNF-α预包被ELISA(酶联免疫吸附测定)试剂盒是一种固相免疫测定法,其特别设计用于用96孔带板测量人TNF-α,其预涂覆有针对TNF的小鼠单克隆抗体-α。它含有大肠杆菌表达的重组人TNF-α。检测抗体是特异于TNF-α的生物素化山羊多克隆抗体。该试剂盒经过现成的试剂分析验证。

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution.

The BioGems Human TNF-α Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNF-α with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TNF-α. It contains E. coli-expressed recombinant Human TNF-α. The detection antibody is a biotinylated goat polyclonal antibody specific for TNF-α. The kit is analytically validated with ready to use reagents.

Product Details
Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Human TNF-α
Uniprot:
P01375
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.
Components

Description

Quantity

Volume

Anti-Human TNF-α Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human TNF-α Standard

2

10 ng vial

Human TNF-α Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

93

327

608

102

319

613

Standard Deviation

5.1

15.4

31

7.65

15.3

35

Consistency (%CV)

5.5

4.7

5.1

7.5

4.8

5.7

共(0)条评论用户评论

  • 暂时还没有任何用户评论
总计 0 个记录,共 1 页。 第一页 上一页 下一页 最末页
用户名: 匿名用户
E-mail:
评价等级:
评论内容:
  • test
  • tews2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • 9
  • 10
  • 11
  • 12
  • 13
  • 14
  • 15
  • 16