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Human IFN-Gamma Pre-Coated ELISA Kit试剂盒 人IFN-γ ELISA试剂盒

Human IFN-Gamma Pre-Coated ELISA Kit试剂盒 人IFN-γ ELISA试剂盒
  • Human IFN-Gamma Pre-Coated ELISA Kit试剂盒 人IFN-γ ELISA试剂盒
英文名称: Human IFN-Gamma Pre-Coated ELISA Kit
产品应用: 它含有大肠杆菌表达的重组人IFN-γ检测抗体是特异于IFN-γ的生物素化山羊多克隆抗体
中国俗称: 人IFN-γ ELISA试剂盒 中国简称: 人IFN-γ ELISA试剂盒
注册品牌: Peprotech/Biogems 生产厂家: Peprotech/Biogems
订购货号: BGK01579
规格型号: Kit
保存温度: 4-8度
产品种类: 人IFN-γ ELISA试剂盒
到货时间: 现货1~5天
产品编码: YS0010146
市场价格: ¥0元
本店售价: ¥0元
注册用户: ¥0元
金牌用户: ¥0元
购买数量: 单价单位

产品描述

 产品中文名称:Human IFN-Gamma Pre-Coated ELISA Kit

产品英文名称:
人IFN-γ ELISA试剂盒

品牌:Peprotech/Biogems

货号:BGK01579

购买Peprotech/Biogems ELISA试剂盒送精美礼品一份!

上海华雅思创生物科技有限公司代理的Peprotech/Biogems TNF-Alpha ELISA试剂盒的产品描述:

IFN-γ是主要由活化的Th2细胞以及肥大细胞和NK细胞产生的免疫调节细胞因子。小鼠中靶向缺失的IFN-γ导致Th2细胞发育受损,并表明IFN-γ在胃肠道寄生虫的驱逐中起重要作用。IFN-γ对单核细胞和巨噬细胞发挥抗炎作用,抑制炎症细胞因子如IL-1β,TNF-α,IL-6和IL-8的表达。还已经显示IFN-γ增强B细胞增殖并诱导同种型转换,导致IgE产生增加。阻断IFN-γ活性抑制哮喘的病理生理学。人和鼠IFN-γ是交叉物种反应性的。

BioGems人类IFN-γ预包被的ELISA(酶联免疫吸附测定)试剂盒是一种固相免疫测定法,专门用96孔带条板测量人类IFN-γ,预先用小鼠IFN抗体特异性单克隆抗体-γ。它含有大肠杆菌表达的重组人IFN-γ。检测抗体是特异于IFN-γ的生物素化山羊多克隆抗体。该试剂盒经过现成的试剂分析验证。

IFN-γ is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells.  Targeted deletion of IFN-γ in mice resulted in impaired Th2 cell development and indicated an important role for IFN-γ in the expulsion of gastrointestinal parasites.  IFN-γ exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8.  IFN-γ has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE.  Blocking of IFN-γ activity inhibits the pathophysiology of asthma.  Human and murine IFN-γ are cross-species reactive.

The BioGems Human IFN-γ Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IFN-γ with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IFN-γ. It contains E. coli-expressed recombinant Human IFN-γ. The detection antibody is a biotinylated goat polyclonal antibody specific for IFN-γ. The kit is analytically validated with ready to use reagents.

Product Details
Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<2 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Human IFN-γ
Uniprot:
P01579
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.
Components

Description

Quantity

Volume

Anti-Human IFN-γ Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IFN-γ Standard

2

10 ng vial

Human IFN-γ Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

98.5

264

579

107

315

589

Standard Deviation

5.32        

9.24

22.00

8.667

20.79

31.81

Consistency (%CV)

5.4

3.5

3.8

8.1

6.6          

5.4

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