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Human TGF-β1 Pre-Coated ELISA Kit 人TGF-β1预包被的ELISA试剂盒

Human TGF-β1 Pre-Coated ELISA Kit 人TGF-β1预包被的ELISA试剂盒
  • Human TGF-β1 Pre-Coated ELISA Kit 人TGF-β1预包被的ELISA试剂盒
英文名称: Human TGF-β1 Pre-Coated ELISA Kit
产品应用: 涉及各种生理过程,包括胚胎发生,组织重塑和伤口愈合
中国俗称: 人TGF-β1预包被的ELISA试剂盒 中国简称: ELISA试剂盒
注册品牌: Peprotech/Biogems 生产厂家: Biogems
订购货号: BGK01137
规格型号: 96T
保存温度: 2~8度
产品种类: ELISA kit
到货时间: 现货1~5天
产品编码: YS0010146
市场价格: ¥0元
本店售价: ¥0元
注册用户: ¥0元
金牌用户: ¥0元
购买数量: 单价单位

产品描述

产品中文名称:人TGF-β1预包被的ELISA试剂盒

产品英文名称:Human TGF-β1 Pre-Coated ELISA Kit



品牌:Peprotech/Biogems

货号:BGK01137

名称:Human TGF-β1 Pre-Coated ELISA Kit试剂盒 人TGF-β1预包被的ELISA试剂盒

购买Peprotech/Biogems ELISA试剂盒送精美礼品一份!

上海华雅思创生物科技有限公司代理的Peprotech/Biogems TNF-Alpha ELISA试剂盒的产品描述:

TGF-β,TGF-β1,β2和β3的三种哺乳动物异构体通过相同的受体信号并产生相似的生物反应。它们是调节细胞增殖,生长,分化和运动以及细胞外基质的合成和沉积的多功能细胞因子。它们涉及各种生理过程,包括胚胎发生,组织重塑和伤口愈合。它们主要分泌为潜在的复合物,其存储在细胞表面和细胞外基质中。从潜在复合物释放生物活性的TGF-β同种型涉及复合物的蛋白水解加工和/或蛋白质如血小板反应蛋白-1的构象变化的诱导。TGF-β1是几乎每种细胞类型分泌的最丰富的同种型。

BioGems人类TGF-β1预包被ELISA(酶联免疫吸附测定)试剂盒是一种固相免疫测定法,专门用96孔带条板测量人TGF-β1,预先包被了TGF-β1特异性的小鼠单克隆抗体-β1。它含有大肠杆菌表达的重组人TGF-β1。检测抗体是特异于TGF-β1的生物素化山羊多克隆抗体。该试剂盒经过现成的试剂分析验证。

The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors.

The BioGems Human TGF-β1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TGF-β1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TGF-β1. It contains E. coli-expressed recombinant Human TGF-β1. The detection antibody is a biotinylated goat polyclonal antibody specific for TGF-β1. The kit is analytically validated with ready to use reagents.

测定类型:固相夹心ELISA:96孔条板
灵敏度:<1 pg / ml
范围:15.6pg / mL-1,000pg / mL
特异性:天然和重组人TGF-β1
如Uniprot:P01137
干扰:与其他相关蛋白质没有可检测的交叉反应性
储存条件:将未开封的产品储存在2 - 8°C。

Product Details
Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Human TGF-β1
Uniprot:
P01137
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.
Components

Description

Quantity

Volume

Anti-Human TGF-β1 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human TGF-β1 Standard

2

10 ng vial

Human TGF-β1 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

347

1638

2586

511

1825

3120

Standard Deviation

13.88

85.18

163

29.64

100.4

246.5

Consistency (%CV)

4

5.2

6.3

5.8

5.5

7.9

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