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Human IL-1β Pre-Coated ELISA Kit人IL-1β预包被的ELISA试剂盒

Human IL-1β Pre-Coated ELISA Kit人IL-1β预包被的ELISA试剂盒
  • Human IL-1β Pre-Coated ELISA Kit人IL-1β预包被的ELISA试剂盒
英文名称: Human IL-1β Pre-Coated ELISA Kit
产品应用: 诱导IL-2释放刺激胸腺细胞增殖,B细胞成熟和增​​殖,促有丝分裂的FGF样活性,以及​​从滑膜细胞释放前列腺素和胶原酶
中国俗称: IL-1β ELISA试剂盒 中国简称: IL-1β 试剂盒
注册品牌: Peprotech/Biogems 生产厂家: Peprotech/Biogems
订购货号: BGK01584
规格型号: BGK01584
保存温度: 4-8度
产品种类: ELISA 试剂盒
到货时间: 现货1~5天
产品编码: YS0010146
市场价格: ¥0元
本店售价: ¥0元
注册用户: ¥0元
金牌用户: ¥0元
购买数量: 单价单位

产品描述

产品中文名称:人IL-1β预包被的ELISA试剂盒

产品英文名称:Human IL-1β Pre-Coated ELISA Kit



品牌:Peprotech/Biogems

货号:BGK01584

名称:Human IL-1β Pre-Coated ELISA Kit试剂盒 人IL-1β预包被的ELISA试剂盒

购买Peprotech/Biogems ELISA试剂盒送精美礼品一份!

上海华雅思创生物科技有限公司代理的Peprotech/Biogems Human IL-1β Pre-Coated ELISA Kit试剂盒的产品描述:

IL-1β是在各种细胞中产生的促炎细胞因子,包括单核细胞,组织巨噬细胞,角质形成细胞和其他上皮细胞。IL-1α和IL-1β都与相同的受体结合,并且具有相似的,如果不是相同的生物学特性。这些细胞因子具有广泛的活性,包括通过诱导IL-2释放刺激胸腺细胞增殖,B细胞成熟和增​​殖,促有丝分裂的FGF样活性,以及​​从滑膜细胞释放前列腺素和胶原酶。然而,IL-1β是分泌的细胞因子,IL-1α主要是与细胞相关的细胞因子。

BioGems人类IL-1β预包被ELISA(酶联免疫吸附测定)试剂盒是一种固相免疫测定法,专门用96孔带板测量IL-1和β,预先包被有IL- 1&beta版。它含有大肠杆菌表达的重组人IL-1和β。检测抗体是特异于IL-1和β的生物素化山羊多克隆抗体。该试剂盒经过现成的试剂分析验证。

IL-1β is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells.  Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties.  These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells.  However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.

The BioGems Human IL-1β Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-1&beta with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-1&beta. It contains E. coli-expressed recombinant Human IL-1&beta. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-1&beta. The kit is analytically validated with ready to use reagents.

上海华雅思创生物科技有限公司代理的Peprotech/Biogems Human IL-1β Pre-Coated ELISA Kit试剂盒测定类型:

测定类型:固相夹心ELISA:96孔条板
灵敏度:<.15pg / ml
范围:3.9 pg / mL - 250 pg / mL
特异性:天然和重组人IL-1β
如Uniprot:P01584
干扰:与其他相关蛋白质没有可检测的交叉反应性
储存条件:将未开封的产品储存在2 - 8°C。

Components

Description

Quantity

Volume

Anti-Human IL-1β Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-1β Standard

2

1 ng vial

Human IL-1β Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

19

41.2

116

27.5

61.3

179.1

Standard Deviation

1

2.6

6.5

1.6

4.1

12.7

Consistency (%CV)

5.3

6.3

5.6

5.8

6.7

7.1

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