产品英文名称：Porcine and Canine Insulin ELISA kit
§ 检测过程：各添加25μl标准物，对照物和样品→加75µl配体物→微孔板摇床温育2小时，700－900rpm→清洗缓冲液清洗6次→加100µl TMB作用物→微孔板摇床温育15分钟，700－900rpm→添加100µl反应终止液→分光光度计450nm读数
ALPCO Porcine and Canine Insulin ELISA kit英文简述：
The ALPCO Porcine and Canine Insulin ELISA kit is for the quantification of porcine or canine insulin concentrations in serum and plasma samples. Utilizing a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips, a single Porcine and Canine Insulin ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time. Additional information regarding the Porcine and Canine Insulin ELISA kit can be accessed by clicking on the “Protocol” link or “Support” tab above.
The ALPCO Insulin (Porcine/Canine) ELISA is for the quantitative determination of insulin in Porcine/ Canine serum and plasma.
PRINCIPLE OF THE ASSAY
The ALPCO Insulin (Porcine/Canine) ELISA is a sandwich type immunoassay. Mouse monoclonal antibodies specific for insulin are immobilized to the 96-well microplate as the solid phase. Standards, controls, and samples are added to the appropriate wells with a horseradish peroxidase enzyme labeled monoclonal antibody (Conjugate), resulting in insulin molecules being sandwiched between the solid phase and the Conjugate. After incubation on a microplate shaker at room temperature, the microplate wells are washed with Wash Buffer to remove unbound Conjugate. TMB Substrate is added to each well, and the microplate is again incubated on a microplate shaker at room temperature. During the second incubation, a blue color results from TMB Substrate reacting with bound Conjugate in the wells. Stop Solution is added and this stops the reaction and changes the color from blue to yellow. The optical density (OD) is measured by microplate reader at 450 nm with a reference wavelength of 620 nm. The intensity of the color generated is directly proportional to the amount of insulin in the sample.